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Public Health Laboratory

    

Contact Information
Los Angeles County
Department of Public Health
Public Health Laboratory
12750 Erickson Avenue
Downey, California 90242

Laboratory Director
Nicole M. Green, Ph.D, D(ABMM)

Phone: (562) 658-1300
Fax: (562) 401-5999

Monday - Friday
8:00 AM to 5:00 PM
Saturday
8:00 AM to 4:30 PM
Public Health Laboratory - Laboratory Testing Information
PHL Code Test Name Test Comment Reference Range Specimen Requirements Set Up Schedule TAT CPT Code
0290 ACID-FAST BACILLI, AMPLIFIED MYCOBACTERIUM TUBERCULOSIS DIRECT (MTD) Test performed when qualified by TB Control and Public Health Laboratory Guidelines. Not available as a routine test. By Report Properly collected sputum. Mon, Wed, Fri 1-4 days 87015, 87556
0101 ACID-FAST BACILLI CULTURE AND SMEAR Standard reference and fast track procedures are used for culturing and staining of all Mycobacteria. Smear: No acid-fast bacilli seen

Culture: No Mycobacterial species isolated
Clinical specimen properly collected. Refrigerate. Daily, Mon-Sat Smear 1 day Culture 1-8 wks 8 wks –negative 87206: Smear 87116, 87015
0102 ACID-FAST BACILLI IDENTIFICATION HPLC, nucleic acid probes and conventional methods are used for identification. Probes for M. tuberculosis complex, M. avium, M. intracellulare, M. gordonae and M. kansasii are available. By Report Pure culture HPLC: Mon, Th or: once weekly 1-5 wks 87206, 87116, 87118, 87015
0103 ACID-FAST BACILLI, PRIMARY DRUG SUSCEPTIBILITY PANEL BY BROTH FOR M. TUBERCULOSIS Panel includes: Isoniazid (INH), Ethambutol (EMB), Rifampin (RIF), and Streptomycin (SM) and Pyrazinamide (PZA) Call laboratory TB section to request drug susceptibilities for mycobacteria other than M. tuberculosis. [EIA Result Entry]![EIA Result] Pure culture Mon, Th 2-3 wks 87190: Ea drug
0104 ACID-FAST BACILLI, PYRAZINAMIDE (PZA) SUSCEPTIBILITY TESTING BY BROTH FOR M. TUBERCULOSIS   By report Pure culture Mon, Th 2-3 wks 87190: Ea drug
0105 ACID-FAST BACILLI, CUSTOM DRUG SUSCEPTIBILITY PANEL BY BACTEC FOR MYCOBACTERIA   By Report Pure culture Mon 2-3 wks 87190: Ea drug
0106 ACID-FAST BACILLI, DRUG SUSCEPTIBILITY PANEL BY AGAR PROPORTION METHOD   By Report Pure culture      
0107 ACID-FAST BACILLI SMEAR, “ONLY” Standard reference and fast track procedures are used for staining of all Mycobacteria. Smear: No acid-fast bacilli seen Clinical specimen properly collected. Refrigerate. Daily, Mon-Sat Smear 1 day 87015, 87206: Smear
0280 ADENOVIRUS CULTURE, SEE VIRAL CULTURE COMPREHENSIVE   Negative Appropriate clinical specimen. (See Virology Information) Daily, Mon-Sat 2 wks (negative) 87252, 87253
0110 AEROBIC BACTERIAL CULTURE, ROUTINE All cultures are performed by standard reference bacterial procedures. Routine specimens other than stool (throat, sputum, skin, eyes etc.) are included. Normal flora Clinical specimen, properly collected and submitted in transport system. Refrigerate. Daily, Mon-Sat 2 days (Negative) 87077
0111 AEROBIC BACTERIAL CULTURE, SPECIAL Please indicate suspected organism(s) when submitting specimen. Listeria monocytogenes, Vibrio Cholerae Other None isolated Clinical specimen, properly collected and submitted in transport system. Refrigerate. If submitting inoculated culture media, hold at room temperature. Daily, Mon-Sat 5 days 7 (Negative) 87070
0113 AEROBIC BACTERIAL CULTURE, STOOL, CAMPYLOBACTER ONLY   No Campylobacter species isolated 1-2 gm of stool (size of thumbnail) in Para Pak container Daily, Mon-Sat 4 days (Negative) 87046
0114 AEROBIC BACTERIAL CULTURE, STOOL, SALMONELLA AND SHIGELLA ONLY   No Salmonella or Shigella species isolated 1-2 gm of stool (size of thumbnail) in Para Pak container Daily, Mon-Sat 5 days (Negative) 87045, 87046, 87077
0115 AEROBIC BACTERIAL CULTURE, STOOL, YERSINIA ENTEROCOLITICA ONLY   No Yersinia enterocolitica isolated 1-2 gm of stool (size of thumbnail) in Para Pak container Daily, Mon-Sat 4 days (Negative) 87046
0305 AEROBIC BACTERIAL CULTURE, STOOL, VIBRIO SPECIES ONLY   No Vibrio species isolated 1-2 gm of stool (size of thumbnail) in Para Pak container Daily, Mon-Sat 4 days (Negative) 87046, 87147, 87077
0118 AEROBIC BACTERIAL IDENTIFICATION   By report Pure culture Daily, Mon-Sat 5 days 87077, 87147
0119 AEROBIC SUSCEPTIBILITY BY DISK DIFFUSION METHOD Studies performed on FDA approved drugs. By report Pure culture Daily, Mon-Sat 2 days 87184
0120 ANAEROBIC BACTERIAL CULTURE, ROUTINE   No anaerobes isolated Clinical specimen properly collected and transported under anaerobic conditions Daily, Mon-Sat 3 days (Negative) 87075
0121 ANAEROBIC BACTERIAL IDENTIFICATION   By report Pure culture Daily, Mon-Sat 4 days 87076
0122 ARBOVIRUS ANTIBODY PANEL, IFA, SERUM Serum is tested against antigens of St. Louis Encephalitis and Western Equine Encephalitis viruses. IgG titers of ≥16 suggest exposure, while the presence of IgM indicates recent infection. Incidence is seasonal from April to November. See specific arbovirus for individual testing. IgG <16 IgM <16 1 ml serum (minimum 0.2 ml) Tu, Th, Fri 2 days 86653, 86654
0123 ARBOVIRUS ANTIBODY PANEL, IFA, CSF   IgG <1 IgM <1 1 ml CSF (minimum 0.2 ml) Tu, Th, Fri 2 days 86653, 86654
0129 BABESIOSIS DETECTION, BLOOD SMEAR (GIEMSA STAIN)   None detected 7 ml whole blood in EDTA (lavender top) tube. Submit to Laboratory within 24 hours or prepare 1 thick and 1 thin smear. Daily, Mon-Sat 1 day 87177, 88313
0131 BORDETELLA PERTUSSIS/PARAPERTUSSIS CULTURE AND DFA. DFA is performed on clinical specimens and reported as a preliminary result. Cultures on Regan Lowe agar are checked for up to 14 days before reporting no growth. DFA: Negative CULTURE: No B. pertussis/ parapertussis isolated. Nasopharyngeal swab submitted on Regan Lowe Medium. Daily, Mon-Sat Culture 14 days DFA 1 day 87081; Cult, 87265, DFA, 87158
0132 BORDETELLA PERTUSSIS/PARAPERTUSSIS CULTURE Culture on Regan Lowe agar is checked for up to 14 days before reporting no growth. No B. pertussis/ parapertussis isolated. Nasopharyngeal swab submitted on Regan Lowe Medium. Daily, Mon-Sat 14 days 87081, 87158, 87077
0133 BORDETELLA PERTUSSIS/PARAPERTUSSIS ANTIGEN DETECTION, DFA   Negative Nasopharyngeal swab in Regan Lowe Deep Medium or an air-dried and heat fixed smear for the DFA procedure. Daily, Mon-Sat 1 day 87265
0135 BRUCELLA IDENTIFICATION   By report Pure culture Daily, Mon-Sat 5-7 days 87070
0138 CHLAMYDIA TRACHOMATIS CULTURE (For Medico-Legal Case) Specimens are plated after centrifugation onto cyclohexamide treated McCoy cell monolayers. Cultures are stained after 48 hours with polyclonal antisera for inclusion bodies characteristic of C. trachomatis. Negative Use M4-RT Transport Medium. Refrigerate Please transport to PHL within 24 hours on ice. Daily, Mon - Sat 3 days 87110, 87207
0140 CHLAMYDIA TRACHOMATIS, NUCLEIC ACID AMPLIFICATION, APTIMA Combo 2 Assay   Negative Cervical or male urethral swab in APTIMA Combo 2 Assay Unisex Swab Specimen Collection Kit. 2 mL of mixed urine in the APTIMA Combo 2 urine specimen transport tube. Store and transport at 2° to 30°C. Daily, Mon-Sat 3 days 83898, 87491
0141 CLOSTRIDIUM BOTULINUM CULTURE   No Clostridium botulinum isolated Consult with BioTerrorism Response Unit for specimen requirements. Daily, Mon-Sat 5-12 days 87075
0142 CLOSTRIDIUM BOTULINUM NEUTRALIZATION TEST   Negative Consult with BioTerrorism Response Unit for specimen requirements. Daily, Mon-Sat 4 days Additional 24-48 hours if dialysis required. 87001, 87230
0143 COCCIDIOIDES ANTIBODY, CF, SERUM All serum titers ≥4 should be considered presumptive evidence of coccidioidomycosis. Titers exceeding 16 usually reflect disseminated disease. In general, the higher the titer, the more severe the disease, and changes in serial titers are also of prognostic value. A negative CF test does not, however, rule out the diagnosis. Patients with cavitary disease are only 70% positive, and with nodular diseases, only 30% positive. <4 1 ml serum (minimum 0.2 ml) Th 8 days 86171
0144 COCCIDIOIDES AB, CF, CSF   <1 1 ml CSF (minimum 0.2 ml) Th 8 days 86171
0145 COCCIDIOIDES AB, IgG AND IgM, EIA, SERUM A negative result with both IgM and IgG indicates that antibody for C. immitis antigen is either absent, below the level of detection of the assay or the specimen was obtained too early in the response. A positive result with either IgM or IgG implies the presence of antibody to C. immitis antigens. An early acute phase patient may only present an IgM response, while the chronic or convalescent patient may only present an IgG response. Reflex testing to the CF assay will be performed for those patients with history of anibody to Coccidioides antigen. ≤0.149 1 ml serum (minimum 0.2 ml) Daily, Mon-Fri 2 days 86635(x2)
0146 COCCIDIOIDES AB, IgG AND IgM, EIA, CSF   ≤0.149 1 ml CSF (minimum 0.2 ml) Mon-Fri 2 days 86635(x2)
0147 CORYNEBACTERIUM DIPHTHERIAE CULTURE     Call the Bacteriology Section for specific requirement Daily, Mon-Sat 5 days 7 days (negative) 87070
  COXSACKIE B ANTIBODY NEUTRALIZATION, SERUM   By Report 2 ml serum Mon-Fri 3 wks 86382
0148 CRYPTOSPORIDIUM/GIARDIA, STOOL, DFA This procedure is an in vitro DFA for the simultaneous detection of Cryptosporidium oocysts and Giardia cysts in fecal material. None detected Stool in 10% Formalin Daily, Mon-Sat 1-2 days 87272
0171 CRYPTOSPORIDIUM / ISOSPORA / CYCLOSPORA DETECTION, DIRECT STAIN (MODIFIED ACID FAST STAIN)   None detected Stool in 10% Formalin Daily, Mon-Sat 1-2 days 87015, 87206
0156 CYTOMEGALOVIRUS (CMV) CONVENTIONAL AND RAPID CULTURE   Negative Appropriate specimen (See Virology Information) Daily, Mon-Sat 4 wks (negative) 87252 (x2), 87198, 87253
0157 E. COLI 0157:H7 SEROTYPING, SHIGA TOXIN POSITIVE STRAIN E. coli 0157:H7 has emerged as an enteric pathogen of public health importance, causing multiple outbreaks of hemorrhagic colitis, hemolytic uremic syndrome and diarrhea in nursing homes, day care centers, schools, and the community. One or two cytotoxins may be elaborated. Toxin producing E. coli of various serotypes has been reported so the syndrome is not limited to E. coli 0157:H7 None detected Pure culture of E. coli Daily, Mon-Sat 4 – 14 days 87046, 87147, 87335
0158 E. COLI 0157:H7, STOOL CULTURE SCREEN   No E. coli:0157 isolated 1 gram stool in Para Pak container Daily, Mon-Sat 3 days 87046 (x2)
0159 E. COLI SHIGA TOXIN DETECTION   None detected Pure culture of E. coli Daily, Mon-Sat 3 days 87147, 87335
0204 ECTOPARASITE IDENTIFICATION   By report Place intact arthropod in a 15ml centrifuge tube. Seal cap securely. Daily, Mon-Sat 1-2 days 87168
0209 ENTAMOEBA HISTOLYTICA, EIA, STOOL The entamoeba histolytica test is an enzyme immunoassay for the rapid detection of the adhesin of E. histolytica in human fecal specimens. This test distinguishes E. histolytica from E. dispar whereby when diagnosis is made by light microscopy these protozoans are indistinguishable. Negative ≤ 0.490 Unpreserved stool specimen. Must be frozen if unable to deliver to the laboratory within 12 hours. Mon, Wed, Fri 1-2 days 87337
0162 ENTEROVIRUS TYPING   By report Submit enterovirus isolate Mon-Fri 3 wks 87252, 87253, 87199
0163 EPIDEMIOLOGY: MOLECULAR STRAIN TYPING ANALYSIS USING PULSED-FIELD GEL ELECTROPHORESIS (PFGE) PFGE is used for the molecular strain typing of selective organisms. By report Consult with the Enteric Bacteriology Section before submitting specimens Mon Consult Molecular Epidemiology Section 87152, 83891, 83892, 83894, 83912
0164 EPIDEMIOLOGY: DNA FINGERPRINTING OF MYCOBACTERIUM TUBERCULOSIS USING RESTRICTION FRAGMENT LENGTH POLYMORPHISM (RFLP) ANALYSIS DNA fingerprinting by RFLP has proven to be a valuable strain typing technique used for epidemiologic investigations of suspected outbreaks, reinfections vs. reactivation of infection, laboratory cross contaminations, and laboratory errors. By report Consult with the Molecular Epidemiology Section before submitting specimens Mon Consult Molecular Epidemiology Section 87116, 87118, 83891, 83892, 83894, 83896, 83897, 83912
0297 FECAL COLIFORMS, WATER   By report Consult with the Waters Section Mon-Fri 2-3 days 87999
0176 FUNGAL CULTURE AND IDENTIFICATION   None isolated Properly collected clinical specimen Daily, Mon-Fri 4 wks (Negative) 8 wks – if dimorphic fungus suspected 87106, 87107, 87206, 87210 87015 – sputum 87176 – tissue
0179 FUNGAL IDENTIFICATION   By report Pure culture Daily, Mon-Fri Consult Mycology Lab 87106 – yeast 87107 – mold
FUNGAL IDENTIFICATION, DNA PROBE FOR COCCIDIOIDES IMMITIS OR HISTOPLASMA CAPSULATUM   Negative Pure culture Mon 7 days 87797
0180 HAEMOPHILUS INFLUENZAE SEROTYPING (A-F), SLIDE AGGLUTINATION   By report Pure culture on chocolate slant Daily, Mon-Sat 5 days 87147
0182 HEPATITIS A IgM ANTIBODY, EIA, SERUM A positive result indicates recent exposure to HAV. The IgM response will start approximately 2 months after exposure and continues to be measurable for up to 8 months after exposure. Negative 1 ml serum (minimum 0.5 ml) T, Th 4 days 86709
0306 HEPATITIS A TOTAL ANTIBODY, EIA, SERUM The presence of anti-HAV is indicative of past or present infection with Hepatitis A Virus. Anti-HAV is detectable during the acute stage of illness (anti-HAV IgM) and may persist for years after recovery. The test for total anti-HAV is primarily used to determine previous exposure to Hepatitis A Virus and may be used to assess immune status. Nonreactive 2.0 ml serum (minimum 1.5 ml) T, Th, Fri 4 days 86708
0183 HEPATITIS B CORE ANTIBODY, EIA, SERUM Hepatitis B core antibody (HBcAb) is present in serum after the appearance of hepatitis B surface antigen (HBsAg) and before the appearance of hepatitis B surface antibody (HBsAb). In the absence of HBsAg and HBsAb, HBcAb may be the only serologic marker of recent hepatitis B infection and potentially infectious blood. Nonreactive 2 ml serum (minimum 1 ml) T, Wed, Th, Fri 2 days (negative) 86704
0184 HEPATITIS B SURFACE ANTIBODY, EIA, SERUM   Nonreactive 1 ml serum (minimum 0.5 ml) T, Wed, Th, Fri 2 days 86706
0185 HEPATITIS B SURFACE ANTIGEN, EIA, SERUM The hepatitis B surface antigen (HBsAG) is present in acute hepatitis B infection or in chronic carriers of hepatitis B. HBsAG becomes detectable after the incubation stage of the virus that generally lasts 4-12 weeks after exposure. Negative 2.0 ml serum (minimum 1.5 ml) T, Wed, Th, Fri 2 days (negative) 87340
0190 HEPATITIS C ANTIBODY, EIA, SERUM   Nonreactive 2 ml serum T, Wed, Th, Fri 2 days (negative 86803
0196 HERPES SIMPLEX VIRUS (HSV), NON-GENITAL LESION SPECIMEN SOURCE OR FOR LEGAL ISSUES, CONVENTIONAL CULTURE   Negative Appropriate specimen (See Virology Information) Daily, Mon-Sat 14 days (negative) 87207, 87252, 87253, 87273, 87274
0197 HERPES SIMPLEX VIRAL DNA (HSV) REAL-TIME PCR DETECTION   Negative Appropriate specimen (See Virology Information) Daily, Mon-Fri 1 day 87273, 87274, 87801
0194 HERPES SIMPLEX VIRUS TYPE 2 (HSV-2), IgG, EIA, SERUM The HSV 2 assay is intended for the qualitative detection of the presence of absence of human IgG antibodies to HSV-2 in human sera. HSV-2 is primarily associated with genital and neonate infection. When the primary HSV-2 infection is clinical, the classical presentation is herpes genitalis, an infection characterized by bilaterally distributed lesions in the genital area accompanied by fever, inguinal lymphadenopathy and dysuria. HSV-2 infections cause approximately 85% of symptomatic primary genital HSV cases and 99% of recurrent genital herpes. Negative 1 ml serum (minimum 0.25 ml) Mon 8 days 86696, 86695
0293 HIV-1, ANTIBODY, WESTERN BLOT, ORAL FLUID   Negative Requires special collection device. Contact HIV Serology Unit for information. 3.0 ml plasma (minimum) Daily, Mon-Sat 3 days 86689
0308 HIV-1 GENOTYPING Analysis of DNA sequence data to identify HIV-1 resistance associated mutations using the Bayer Health Care sequencing system; resistance associated mutations are based on current International AIDS Society-USA panel reporting recommendations. Wildtype HIV-1 DNA sequences 3.0 ml plasma (minimum) Specimen must be collected in K2EDTA or Plasma Preparation Tubes (PPT). Separate plasma from whole blood within 6 hours or 2 hours for PPT tubes. For long-term storage freeze 1 ml aliquots at ≤ -20°C (preferably -70°C). Genotyping specimens must have a viral load of ≥1000 copies/ml. Daily, Mon-Fri 14 days 87901
0200 HIV-1 IgG ANTIBODY, WESTERN BLOT, SERUM   Negative 1 ml serum (minimum 0.25 ml) Daily, Mon-Sat 3 days 86689
0291 HIV-1 STANDARD, PCR, QUANTITATIVE VIRAL LOAD TEST An in vitro nucleic acid amplification of human immunodeficiency virus Type 1 (HIV-1) RNA in human plasma. The test is intended for use in prognosis procedures or to monitor the effects of therapy only. This is not a screening or diagnostic test for HIV infection. Lower limit of quantification: 400 copies/ml. Max. detection range: 750,000/ml. Specimen must be collected in K2EDTA. Separate plasma from whole blood within 6 hrs. Transfer plasma to sterile polypropylene tube. Store at -20° to -80° C. Deliver to lab within 5 days. Minimum 2 ml. Daily, Mon-Sat 5 days 87536
0292 HIV-1, SCREENING ANTIBODY, ELISA, ORAL FLUID   Negative Requires special collection device. Contact HIV-Serology unit for information. Daily, Tu-Sat 3 days 86701
0202 HIV-1 SCREENING ANTIBODY, ELISA, SERUM Initial testing is done with ELISA techniques using a whole virus lysate antigen/ synthetic peptides. Positive sera are confirmed by the Western blot. Negative 1 ml serum (minimum 0.1 ml) Daily, Mon-Fri 3 days 86701
0173 HIV-1 ULTRASENSITIVE PCR QUANTITATIVE VIRAL LOAD TEST An in vitro nucleic acid amplification of human immunodeficiency virus Type 1 (HIV-1) RNA in human plasma. The test is intended for use in prognosis procedures or to monitor the effects of therapy only. This is not a screening or diagnostic test for HIV infection. Lower limit of quantification: 50 copies/ml. Max: detection range: 100,000/ml. copies Specimen must be collected in K2EDTA. Separate plasma from whole blood within 6 hrs. Transfer plasma to sterile polypropylene tube. Store at -20 to -80° C. Deliver to lab within 5 days. Minimum 2 ml. Mon-Sat 5 days 87536
0207 LEAD Analysis on whole blood is done using the Graphite Furnace Atomic Absorption Spectrometry <10 ug/dl 250 ul whole blood in EDTA for fingerstick 1 ml whole blood in EDTA or Heparin for venipunture. Daily, Mon-Fri 3 days 83655
0210 LEGIONELLA CULTURE AND DIRECT ANTIGEN DETECTION, DFA   Negative Clinical specimen. Refrigerate Daily, Mon-Sat Culture 7 days DFA 1 day 87081, 87140, 87205, 87206, 87278
0214 MEASLES (RUBEOLA), IgG AB, EIA, SERUM Available only for potential outbreak situations. Not a routine test. ≤0.90 1 ml serum (minimum 0.25 ml) Daily, Mon-Fri 2 days 86765
0215 MEASLES (RUBEOLA) IgG AND IgM ANTIBODY PANEL, IFA, SERUM Available only for potential outbreak situations. Not a routine test. The traditional serologic diagnosis of measles requires a significant rise in antibody titer between acute and convalescent phase sera. However, the diagnosis can also be supported by demonstrating the presence of IgM antibody in a single specimen. Correct interpretation of serologic data depends upon the proper timing of specimen collection in relation to rash onset. This timing is especially important for interpreting negative IgM results since IgM antibody peaks approximately 10 days after exposure and may be undetectable 30 days after rash onset. Asymptomatic reinfection can occur in persons who have previously developed antibodies, whether from vaccination or from natural disease. Symptomatic reinfections are rare. These reinfections have been accompanied by rises in measles antibody titers. IgG: <8 IgM: <10 1 ml serum (minimum 0.25 ml) Daily, Mon-Fri 2 days 86765
0216 MEASLES (RUBEOLA) IgG ANTIBODY, IFA, IMMUNITY SCREEN, SERUM Available only for potential outbreak situations. Not a routine test. IgG: <8 IFA 1 ml serum (minimum 0.25 ml) Daily, Mon-Fri 2 days 86765
0217 MEASLES (RUBEOLA) IgM ANTIBODY, IFA, SERUM Available only for potential outbreak situations. Not a routine test. <10 1 ml serum (minimum 0.25 ml Daily, Mon-Fri 1 day 86765
0150 MICROSPORIDIUM (MODIFIED TRICHROME STAIN)   By report Stool in 10% Formalin Daily, Mon-Fri 3 days 87015, 87207
0227 NEISSERIA GONORRHOEAE CULTURE   None isolated Properly inoculated MTM agar pill pocket plate. Incubate plates until transport. See specimen requirements. Daily, Mon-Sat 3 days 87081, 87140
0294 NEISSERIA GONORRHOEAE, NUCLEIC ACID AMPLIFICATION, APTIMA Combo 2 Assay.   Negative Cervical or male urethral swab in APTIMA Combo 2 Assay Unisex Swab Specimen Collection Kit. 2 mL of mixed urine in the APTIMA Combe 2 urine specimen transport tube. Store and transport at 2° to 30°C. Daily, Mon- Fri 3 days 87591
0301 NEISSERIA GONORRHOEAE NAAT DETECTION Amplified-DNA probe test to screen for the presence of Neisseria gonorrhoeae from throat and rectal swab specimens. Negative Use swabs from the Aptima Vaginal swab specimen Collection Kit to collect throat or rectal specimen. Transport at room temperature within 30 days of collection. Mon - Fri 3 days 87591
0228 NEISSERIA MENINGITIDIS SEROGROUPING   By report Pure culture on chocolate agar slant Daily, Mon-Sat 1 day 87077: Per serogroup
0229 NOCARDIA CULTURE   None isolated Properly collected clinical specimen Daily, Mon-Fri 3 wks (negative) 87077
0294 NOROVIRUS/VIRAL GASTROENTERITIS RT-PCR, STOOL   By report Fresh stool collected in clean dry container, within 48-72 hours after onset of illness. Store at 4°C until delivered to the laboratory. Wednesday Consult Molecular Epidemiology Section 83891, 83902, 83898, 83894, 83912
0231 OVA AND PARASITE EXAMINATION, BLOOD, LIGHT MICROSCOPY Giemsa stain examined for all blood parasites. By report Collect blood in 7ml EDTA (purple top) tube Submit to lab within 24 hours or prepare one thick and one thin smear, Giemsa stained or unstained. Daily, Mon-Sat 1 day 87205, 87207, 87177, 88313, 87015
0232 OVA AND PARASITE EXAMINATION, SPUTUM, LIGHT MICROSCOPY Direct examination of specimen for parasites By report Collect sputum in sterile 50 ml centrifuge tube. Daily, Mon-Sat 2 days 87205, 87207
0233 OVA AND PARASITE EXAMINATION, STOOL, LIGHT MICROSCOPY Trichrome stain and wet mount examined for intestinal parasites By report Stool preserved with ZN-PVA. Transfer fresh stool to fill line on PVA vial. Mix well. Daily, Mon-Sat 2 days 87177, 88313
0234 OVA AND PARASITE EXAMINATION, URINE, LIGHT MICROSCOPY Direct examination of urine sediment for parasites By report Collect first morning voided specimen in sterile 50 ml centrifuge tube. (minimum 15 ml) Daily, Mon-Sat 2 days 87177, 88313
0237 PINWORM PREPARATION, LIGHT MICROSCOPY Specimen is examined for the presence of Enterobius vermicularis. By report Swube or Scotch Tape slide preparation of perianal region Daily, Mon-Sat 1-2 days 87172
0239 POLIOVIRUS ANTIBODY, NEUTRALIZATION, SERUM This sensitive procedure is recommended for vaccine response testing and type-specific serodiagnosis of recent Poliovirus infection. By report 1 ml serum (minimum 0.5 ml) Mon-Fri 3 wks 87252, 87253: Per Serotype
0241 RABIES EXAMINATION, BIOPSY Test includes the fluorescent examination of a specimen smear. Negative Properly collected biopsy specimen from brain or fresh animal head. Daily, Mon-Fri Weekends and after hours on emergencies 1 day 87205, 87207, 87003
0244 RESPIRATORY SYNCYTIAL VIRUS (RSV) ANTIGEN DETECTION, EIA   Negative Swab, nasal aspirate. Do not dilute. Daily, Mon-Sat 1 day 87420
0248 RICKETTSIAL DISEASE ANTIBODY PANEL, IFA This panel includes IgG and IgM antigen specific titers against spotted fever group Rickettsia rickettsii (Rocky Mountain Spotted Fever), R. acari (Rickettsial pox) and the typhus fever group Rickettsia typhi (endemic or murine typhus), Rickettsia prowazeekii (epidemic typhus) and Brill-Zinsser disease caused by reactivation of latent R. prowazekii. IgG <64 IgM <64 1 ml serum or CSF (minimum 0.25 ml) Mon, Wed, Fri 2 days 86757
0254 ROTAVIRUS ANTIGEN DETECTION, EIA Rotaviruses are the major cause of epidemic viral gastroenteritis in infants and young children worldwide, with peak incidence between mid-December and mid-February. They are also associated with a severe sporadic gastroenteritis of infants and young children. Negative 1gram stool or stick swab from diaper. Submit in sterile container; no preservatives. Daily, Mon-Sat 1 day 86759
0255 RPR (RAPID PLASMA REAGIN), SERUM The RPR test is a nontreponemal test to detect antibody reactive with lipoidal antigen of T. pallidum. Reactivity of the RPR test does not develop until 1-4 weeks after the appearance of the chancre. Nontreponemal tests are reactive in primary syphilis in 75-90% of cases. In secondary syphilis nearly 100% of cases are reactive with titers generally ³16. In untreated latent syphilis 70% of the cases are reactive. After adequate treatment of primary and secondary syphilis, there should be at least a four-fold decline in titer by 3 months. False positive RPR results occur in 1% to 2% of the normal population, after acute febrile illnesses, after immunizations or during pregnancy. Chronic false-positives are seen in autoimmune diseases (SLE), chronic infections and intravenous drug users. Generally these titers are £8. A reactive RPR result for routine specimens will be confirmed by reflexing to the treponemal antibody, TP-PA assay. Non-reactive 1 ml serum (no CSF accepted) (minimum 0.25 ml) Daily, Mon-Sat 4 days 86592
0257 RUBELLA IgG AND IgM ANTIBODY PANEL, EIA, SERUM Available only for potential outbreak situations. Not a routine test. The detection of IgG antibodies suggests prior exposure to the virus, either natural or vaccine induced. The demonstration of IgM is valuable in detecting active or recent rubella infections. ≤0.90 1 ml serum (minimum 0.3 ml) Tu, Th, Fri 2 days See Each Assay
0258 RUBELLA IgG ANTIBODY, EIA, SERUM Available only for potential outbreak situations. Not a routine test. ≤0.90 1 ml serum (minimum 0.3 ml) Mon, Wed, Fri 2 days 86762
0259 RUBELLA IgM ANTIBODY, EIA, SERUM Available only for potential outbreak situations. Not a routine test. ≤0.90 1 ml serum (minimum 0.3 ml) Tu, Th, Fri 2 days 86762
0260 SALMONELLA SEROGROUPING, GROUPS A-Z, 51-61 AND VI   By report Pure culture on agar slant Daily, Mon-Sat 4 days 87147
0261 SALMONELLA SEROTYPING   By report Pure culture on agar slant Daily, Mon-Sat 4 days 87147
0262 SHIGELLA SEROTYPING   By report Pure culture on agar slant Daily, Mon-Sat 4 days 87147
0390 SPOLIGOTYPING, REVERSED LINE BLOT HYBRIDIZATION   By report Consult with the Molecular Epidemiology Section before submitting specimens. Tu Consult Molecular Epidemiology Section 87116, 87118, 83891, 83898, 83893, 83912
0220 TREPONEMAL ANTIBODY, TP-PA Treponema pallidum-Particle Agglutination (TP-PA) is a treponemal qualitative gelatin particle agglutination assay intended to be used for the detection of Treponema pallidum antibodies in human serum as an aid in the diagnosis of syphilis. It is based on the agglutination of colored gelatin particle carriers sensitized with T. pallidum (Nichols Strain) antigen. Non-reactive Collect blood in 7ml SST tube (tiger top) (Minimum 2 ml) Daily, Tu-Sat 4 days 86781
0271 TRICHOMONAS PREPARATION Test includes direct microscopic examination of specimen for motile trichomonads No trichomonads found Properly collected clinical specimen Daily, Mon-Sat 1 day 87210
0280 VARICELLA-ZOSTER VIRUS IgG AB, EIA SERUM Available only for potential outbreak situations. Not a routine test. <0.90 1 ml serum (minimum 0.25 ml) Daily, Mon-Fri 1 day 86787
0277 VARICELLA-ZOSTER VIRUS IgG ANTIBODY, IFA, SERUM Available only for potential outbreak situations. Not a routine test. <8 1 ml serum (minimum 0.25 ml) Daily, Mon-Fri 1 day 87290
0279 VARICELLA-ZOSTER VIRUS CULTURE   Negative Appropriate clinical specimen. (See Virology Information) Daily, Mon-Sat 2 wks (negative) 87205, 87207, 87252, 87253
0280 VIRAL CULTURE COMPREHENSIVE   Negative Appropriate clinical specimen. (See Virology Information) Daily, Mon-Sat 2 wks (negative) 87205, 87207, 87252, 87253
0281 VIRAL IDENTIFICATION   By report Cell culture tube Daily, Mon-Sat 1-2 wks 87205, 87207, 87252, 87253
0291 WEST NILE VIRUS ANTIBODY, ELISA, SERUM West Nile Virus is a flavivirus, which can cause aseptic meningitis, meningoencephalitis, and encephalitis. Surveillance only. By report 1 ml serum (minimum 0.5 ml) Refrigerate Consult Serology Section 4 days 86790
0288 WORM IDENTIFICATION, GROSS   By report Place suspected worm or worm fragment from urine, stool or other clinical specimen in 50 ml centrifuge tube. Cover with water or saline. Seal lid securely. Daily, Mon-Sat  48 hours 87169
0289 YERSINIA PESTIS (PLAGUE) ANTIBODY, SERUM, PHA/PHI Serum is screened by Passive Hemagglutination (PHA) and if positive confirmed by Passive Hemagglutination Inhibition (PHI). <10 1 ml serum (minimum 0.5 ml) Wed, Th, Fri 3-4 days 86280, 86793
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